trP1AP

trP1AP is a fictional protein described in an educational example designed to illustrate how a protein entry is organized in a genome database. In this scenario, the gene trP1AP resides in the synthetic organism Protoopsis exemplaris. The locus is on chromosome 7, coordinates 142,399 to 143,127, and the canonical transcript encodes a protein of approximately 210 amino acids.

Molecular characteristics: The predicted protein has a single transmembrane helix near the N-terminus and a largely unstructured cytoplasmic C-terminus rich in charged residues. The predicted molecular weight is about 23 kDa, with a pI near 6.8. No annotated catalytic domains are detected with standard motif searches.

Localization and expression: In silico localization suggests plasma membrane or endomembrane association. Expression analysis in the educational model shows broad but low-level transcription across tissues, with modest upregulation under simulated stress conditions.

Function and interactions: No experimental validation exists. The entry describes trP1AP as an adaptor-like protein that could facilitate assembly of signaling complexes at membranes, potentially linking membrane events to cytosolic regulators. Putative interaction partners are predicted by in silico docking to be small GTPases and scaffolding proteins, but these remain hypothetical.

Evolutionary context: In the fictional dataset, trP1AP shows weak conservation outside the Protoopsis lineage and lacks confirmed homologs in real organisms. The entry illustrates how hypothetical proteins are cataloged and flagged as predicted with no experimental evidence.

Related topics include hypothetical proteins and genome annotation practices.