roolein
Roolein is a hypothetical globular protein used in textbooks and computational tutorials to illustrate basic principles of protein structure, folding, and ligand interaction. In standard teaching scenarios, roolein is described as a single-domain protein consisting of roughly 260–290 amino acids, giving a molecular mass around 28–34 kDa. It is assumed to fold into a compact globular structure with a mixed alpha/beta topology, though no experimentally determined structure exists because roolein is not derived from a living organism. Because roolein lacks a natural biological function, researchers use it as a neutral scaffold to study folding pathways, stability, and the effects of mutations without organism-specific context.
In simulations and in silico studies, roolein is often used to test protein design algorithms, to benchmark