mikrodializ
Mikrodializ is a sampling technique used to measure concentrations of soluble molecules in the extracellular fluid of living tissue by diffusion across a semipermeable membrane. A small dialysis probe is implanted into the target tissue. An isotonic perfusate is pumped through the probe at a slow, constant flow. Molecules from the surrounding interstitial fluid diffuse across the membrane into the perfusate and are collected as dialysate for analysis.
Microdialysis enables continuous, in vivo sampling with higher temporal resolution than many traditional methods. This approach
Typical probes use a concentric design with a semi-permeable membrane, and the membrane length is often in
Dialysate is analyzed with various laboratory techniques, including high-performance liquid chromatography, liquid chromatography–mass spectrometry, and capillary
Applications span neuroscience (for example, monitoring dopamine, glutamate, and GABA), metabolism (glucose, lactate), and pharmacokinetics (tissue