basiskalling
Basiskalling, also known as base calling, is the computational process of converting raw signals produced by DNA sequencing instruments into a string of nucleotide bases (A, C, G, T, and sometimes N for unknown). It is a central step in most sequencing workflows, including widely used platforms such as Illumina, Ion Torrent, PacBio, and Oxford Nanopore.
The process typically involves interpreting instrument-specific signals—such as fluorescence intensities or ionic currents—at each sequencing cycle
The primary output of basiskalling is reads in a standard format (often accompanied by per-base quality scores,
Accuracy in basiskalling depends on factors like signal-to-noise ratio, instrument calibration, chemistry, read length, and platform-specific
See also: base calling in sequencing, sequencing quality scores, next-generation sequencing, sequencing platforms, FASTQ formats.