TransformationEvent
TransformationEvent refers to any instance in which genetic material is introduced into a host organism and becomes part of its genome or is expressed. It is a term used across biology, especially microbiology, molecular biology, and biotechnology, to describe the crucial moment when foreign DNA is taken up and integrated or maintained.
In bacteria, transformation involves uptake of naked DNA by competent cells. The transformation event can result
In plant and mammalian cells, transformation events are achieved with methods such as Agrobacterium tumefaciens or
Detection and verification include PCR, sequencing, Southern blot, or RNA expression analysis; transformation events are evaluated
Historical context and applications: The concept traces to Frederick Griffith's 1928 experiments showing that DNA can