Perplot

Perplot is a software tool used for the management and analysis of Droplet Digital PCR (ddPCR) and other quantitative PCR (qPCR) data. It is designed to provide an integrated environment for data analysis, visualization, and interpretation.

Perplot was initially developed to address the need for a comprehensive and user-friendly solution for analyzing ddPCR data. It utilizes algorithms that account for the specific characteristics of ddPCR, such as template copy number variation and primer binding efficiency. This allows for the accurate calculation of absolute quantities and the detection of population variations.

The software supports multiple file formats, including those from major ddPCR instrument manufacturers. Users can import raw data, apply various analysis protocols, and visualize results in a variety of plots and tables. Perplot also includes features for data normalization, heterogeneity analysis, and the detection of mixed populations.

Perplot is widely used in research settings, particularly in fields such as gene expression studies, mutations analysis, and disease research. Its applicability in various qPCR and ddPCR applications has been demonstrated in numerous scientific publications. The software is available for download or through a web application, making it accessible to a broad range of users.

By providing an intuitive interface and a comprehensive set of analytical tools, Perplot accelerates the discovery process in fields that employ quantitative PCR and ddPCR. The software development is ongoing, with regular updates and improvements to incorporate new features and support emerging technologies.

The flexibility and versatility of Perplot have made it a valuable resource in the field of molecular biology and beyond. Its continued development and refinement aim to maintain its position as a reliable and versatile tool for data analysis and interpretation.