Nanopipetták

Nanopipetták, also known as nanoliter pipettes, are specialized laboratory instruments designed for precise liquid handling at the nanoliter scale. These devices are essential in fields such as molecular biology, biochemistry, and nanotechnology, where accurate dispensing of extremely small volumes is critical. Unlike conventional pipettes, which handle microliters or milliliters, nanopipettes are engineered to deliver volumes ranging from a few nanoliters (10⁻⁹ liters) to several hundred nanoliters with high precision.

The primary function of a nanopipette is to transfer, mix, or aspirate minute quantities of liquids with minimal contamination or loss. They often incorporate features such as adjustable tip sizes, variable volume controls, and air-dispensing capabilities to enhance accuracy. Some models utilize electronic or mechanical systems to automate the pipetting process, reducing human error and improving reproducibility. Nanopipettes are typically paired with specialized tips made from materials like glass or plastic, which are designed to minimize dead volume and ensure clean transfers.

Applications of nanopipettes include DNA/RNA manipulation, protein crystallization, cell culture experiments, and the preparation of samples for advanced imaging techniques such as electron microscopy. Their use is also expanding in emerging areas like nanomedicine and synthetic biology, where precise liquid handling is necessary for creating nanostructures or testing novel compounds.

Maintenance and calibration are crucial for maintaining the performance of nanopipettes, as even minor clogs or miscalibrations can compromise experimental results. Regular cleaning with appropriate solvents and periodic testing against known standards help ensure reliability. While nanopipettes offer unparalleled precision, their complexity and cost make them less accessible than conventional pipettes, limiting their use to specialized laboratories.