Mikrotiterikokeet

Mikrotiterikokeet, also known as microplate assays or microtiter plate assays, are laboratory techniques used to measure the activity of enzymes, antibodies, or other biological molecules. These assays are conducted in small wells of a microtiter plate, which typically consists of 96 or 384 wells arranged in a grid pattern. Each well can hold a small volume of liquid, usually ranging from 100 to 300 microliters.

The process begins with the addition of a specific substrate or reagent to the wells, which reacts with the biological molecule of interest. The reaction produces a measurable change, such as a color change, fluorescence, or chemiluminescence. This change is then detected using specialized equipment, such as a spectrophotometer, fluorometer, or luminometer.

Mikrotiterikokeet are widely used in research and clinical settings due to their high throughput, precision, and automation capabilities. They allow for the simultaneous testing of multiple samples, making them ideal for high-throughput screening and large-scale studies. Additionally, the small sample volumes required reduce the amount of material needed for each test, which is beneficial when working with limited samples or expensive reagents.

However, mikrotiterikokeet also have some limitations. The small well volumes can lead to evaporation and contamination issues, and the use of specialized equipment may require additional training and resources. Despite these challenges, mikrotiterikokeet remain a valuable tool in the field of biological research and diagnostics.