retentiontimebased
Retentiontimebased is a term used to describe analytical approaches and data-processing strategies that use retention time (RT) as the primary discriminant for identifying and quantifying analytes in chromatographic instruments. It is commonly applied in liquid chromatography (HPLC/UHPLC) and gas chromatography (GC), as well as in LC–MS workflows where chromatographic separation precedes detection.
In practice, retention time-based methods rely on established RT libraries or predicted RT models. Analysts match
Retention times are influenced by multiple factors including column chemistry, mobile phase composition, temperature, flow rate,
Challenges of retention-time-based approaches include retention time drift over time, co-elution of compounds, and library limitations.
See also: Chromatography, HPLC, UHPLC, GC, LC-MS, retention index, iRT, RT alignment.