radioligandbindingsassays
Radioligand binding assays are biochemical techniques used to study the interaction between a radiolabeled ligand and its binding site, typically a receptor or transporter, in membranes or intact cells. The most common radioligands are radioactive isotopes such as tritium (3H) and iodine-125 (125I). The assay measures specific binding by separating receptor-bound radioligand from free radioligand, usually by filtration or precipitation, followed by quantification of radioactivity with a scintillation counter.
In a typical saturation binding experiment, receptor-rich preparations are incubated with increasing concentrations of radioligand to
Applications include pharmacological characterization of receptors, determination of receptor density in tissues, ligand selectivity profiling, and
Limitations include reliance on radioactive materials, potential artifacts from receptor preparation, and non-specific binding. Safety and