lyseimpuls

Lyseimpuls is a term found in some Nordic-language biological texts to denote a pulse or impulse that induces lysis of cells or microbial cells. The word combines lyse, meaning to rupture, with impuls, meaning impulse. In English-language literature the term is not standard; researchers typically describe lysis methods by mechanism (chemical, enzymatic, mechanical, osmotic, or physical disruption) or by specific technique (electroporation, sonication, bead beating, detergents).

In contexts where lyseimpuls is used, it generally refers to short, energetic pulses intended to compromise cell membranes and release intracellular contents. Electrical pulses, including those associated with electroporation-based lysis or pulsed-field lysing, are common examples; other forms of physical pulses may also be described similarly. The choice of method depends on the organism, matrix, and downstream application.

Applications of lyseimpuls concepts include preparing samples for DNA and RNA extraction, protein recovery, or generating microbial cell lysates for analyses such as PCR, sequencing, or proteomics. The exact pulse parameters—duration, field strength or voltage, and number of pulses—are typically optimized to maximize yield while preserving the integrity of target molecules.

Limitations and considerations include incomplete lysis of some cells, fragmentation of nucleic acids under excessive energy, heat generation, and equipment requirements. Because the term is not universally standardized, authors are advised to refer to the underlying lysis mechanism or specific technique rather than the label alone, ensuring clear communication across languages and laboratories.