cryoSPARC
cryoSPARC is a software platform for cryo-electron microscopy data analysis, with a focus on single-particle analysis and three-dimensional reconstruction. It provides an end-to-end workflow for processing raw cryo-EM micrographs into high-resolution density maps and, when possible, atomic models. The platform emphasizes automated, unsupervised processing to accelerate structure determination and reduce manual intervention.
Key features include ab initio 3D reconstruction, two- and three-dimensional classification, heterogeneous and homogeneous refinement, and
Implementation and usage: cryoSPARC runs on GPU-accelerated hardware and is available as a commercial software package.
Impact: Since its introduction, cryoSPARC has influenced how researchers perform automated, rapid unsupervised structure determination in