The technique underlying atominforcemikroskopia involves a sharp tip attached to the end of a flexible cantilever. As the tip scans over a sample, forces between tip and surface cause the cantilever to deflect. By monitoring this deflection, usually with a laser beam reflected onto a position-sensitive photodetector, the instrument records topographical data and force interactions as a function of position. The deflection data can be processed to produce three-dimensional surface maps, atomic-scale features, or quantitative force curves that provide insight into material properties at the nanometer scale.
Key applications of atominforcemikroskopia include characterizing polymer films, metal oxide surfaces, and biological membranes, as well as evaluating nanocomposites and electronic nanodevices. A significant benefit of this method is its ability to operate under a variety of environmental conditions, from ambient atmosphere to liquid environments, enabling in situ measurements of soft, hydrated samples that would damage under conventional electron microscopy.
Standard operational modes of atominforcemikroskopia include tapping (intermittent contact) and noncontact modes, which minimize damage and reduce artifacts, especially on delicate or soft specimens. These imaging modes also allow simultaneous acquisition of complementary data sets such as phase contrast, elasticity mapping, and friction imaging, broadening the scope of AFM beyond simple surface topography.
With continual improvements in tip fabrication, cantilever design, and software algorithms, atominforcemikroskopia continues to expand its relevance in nanoscale research. Researchers evaluate not only the morphology but also surface chemical composition and mechanical response, making the technique a cornerstone of modern nanoscience and nanotechnology investigations.