Valgusmikroskoopias
Valgusmikroskoopias is a branch of microscopy centered on the use of visible light and glass lenses to magnify and visualize samples at cellular and subcellular scales. It is the most widely used form of microscopy for biological, medical, and materials science investigations. The technique relies on components such as a light source, a condenser, a set of objective lenses, and an eyepiece or camera, with specimens mounted on glass slides and often stained for contrast.
In operation, light is directed to the specimen by the illumination system; the condenser focuses light, the
Abbe's diffraction-based resolution limit applies to valgusmikroskoopias: the finest resolvable detail is roughly 0.2 micrometers under