StoppedflowTechnik
StoppedflowTechnik, or the stopped-flow technique, is a rapid-mixing method used to study fast chemical and biochemical reactions. In this approach, reactants are quickly combined and the flow is stopped within a dedicated observation chamber, allowing time-resolved measurements of the reaction progress.
In a typical setup, solutions are loaded into fast-acting syringes or pumps and injected into a mixing
Applications include pre-steady-state kinetics of enzymes, protein folding, ligand binding, electron transfer, and fast chemical reactions.
Advantages include high time resolution, efficient use of reagents due to small volumes, and direct observation
History and variants: The technique emerged in the 1960s–1970s as a tool for physical chemistry and biophysics