Readpair
Readpair refers to two sequencing reads that originate from opposite ends of the same DNA fragment in paired-end sequencing. In a typical experiment, a single DNA fragment is captured and sequenced from both ends, producing two reads that are collectively called a read pair. Reads are labeled read1 and read2, and they usually face toward each other with an expected insert size corresponding to the length of the fragment between the sequenced ends.
Data for read pairs can be stored in two separate FASTQ files or as interleaved entries in
During analysis, read pairs are mapped to a reference genome in a manner that respects their orientation
Read pairs are central to many sequencing applications, including whole-genome sequencing, transcriptome profiling, and targeted sequencing.