RNasefri

RNasefri, or RNase-free, refers to materials, reagents, surfaces, and environments that are free from ribonucleases, enzymes that degrade RNA. Maintaining RNase-free conditions is essential in molecular biology workflows that involve RNA, because RNases are pervasive, highly stable, and can rapidly compromise RNA samples, affecting experiments such as RT-PCR, RNA sequencing, and Northern blotting.

Achieving RNase-free status involves multiple layers. Laboratories use certified RNase-free consumables (tubes, tips, plates), dedicated equipment and workspaces, and reagents prepared or certified to be free of RNases. Water and buffers are treated or produced under stringent controls (for example, RNase-free water) and surfaces are cleaned with RNase-decontaminating solutions. Practices include wearing gloves, using barrier tips, changing gloves between steps, and separating pre- and post-amplification areas to minimize contamination.

RNase-free products are often labeled as certified RNase-free by manufacturers, and many labs perform tests to verify status. Common verification methods include RNase activity assays or fluorometric indicators that detect residual RNases, as well as checks on RNA integrity in representative samples. Routine validation may accompany supplier certifications and periodic in-house testing.

In practice, RNase-free handling extends to proper storage (keeping reagents sealed and properly refrigerated or frozen), avoiding shared reagents between RNase-free workflows, and maintaining clean, dust-minimized environments. While absolute zero RNases is unachievable, strict adherence to RNase-free principles significantly reduces contamination risk and preserves RNA integrity for sensitive analyses.