RNasefree

RNase-free refers to materials, reagents, equipment, and environments that are treated and maintained to be free of ribonucleases, enzymes that degrade RNA. In molecular biology, RNase-free conditions are essential for experiments that involve RNA, such as RNA extraction, reverse transcription, and RNA sequencing, where even trace RNase contamination can compromise results.

Ribonucleases are widespread and resilient. They can be introduced by human skin, hair, dust, or improperly cleaned surfaces and consumables. RNases may persist in incubators, benches, and pipette tips, making stringent practices necessary to protect RNA integrity. To address this, suppliers offer RNase-free products and certified conditions, including RNase-free water, buffers, tubes, and disposable tips that are treated to remove or inactivate RNases. Some laboratories still use DEPC-treated water, but many rely on commercially certified RNase-free reagents and plastics, often accompanied by batch-specific certification and expiration dates.

Best practices include designating a dedicated work area for RNA work, using separate equipment and consumables, and cleaning surfaces with RNase-decontaminating agents. Laboratory personnel should wear gloves and avoid bringing ink pens or other potential RNase sources into the workspace. Reagents and consumables should be aliquoted to minimize repeated exposure, and samples should be kept on ice when appropriate. RNase-free status is not a guarantee of absolute absence; it represents a controlled state that requires careful handling and routine verification, such as routine testing with RNase-specific assays.

In summary, RNase-free denotes carefully prepared and maintained conditions and materials that help preserve RNA integrity during sensitive molecular biology workflows.