Proteinkanalyser

Proteinkanalyser refer to instruments and methods used to identify, quantify, and characterize proteins in biological samples. They encompass a range of techniques designed to measure concentration, determine molecular weight, assess structure and modifications, and identify specific proteins within complex mixtures. The field spans both traditional biochemistry assays and modern proteomics technologies, from simple colorimetric tests to high-throughput mass spectrometry.

Common techniques include colorimetric and fluorometric assays such as Bradford, BCA, and Lowry for protein quantification; UV-visible spectroscopy for concentration estimates; and electrophoretic methods like SDS-PAGE for size separation. Chromatography, including HPLC and UHPLC, separates proteins or peptides prior to detection. Mass spectrometry, often coupled with liquid chromatography (LC-MS/MS), enables precise identification and characterization of proteins and post-translational modifications. Immunoassays, including ELISA and Western blotting, provide specific detection of target proteins within samples.

Applications of proteinkanalyser span biochemistry, cell and molecular biology, clinical diagnostics, biotechnology, and food science. They are used to quantify biomarkers, study protein expression, analyze proteomes, monitor therapeutic proteins, and assess quality in biopharmaceutical production. Data interpretation relies on appropriate calibration, standard curves, and controls, with attention to matrix effects and instrument performance to ensure accuracy and reproducibility.

Limitations include sensitivity constraints, matrix interferences, and the need for appropriate standards. Ongoing developments aim at increasing throughput, sensitivity, and multiplexing, as well as integrating data analysis with automation and advanced software for proteomics workflows.