Pipetter

Pipetter is a term used in laboratory contexts to refer to a person who performs pipetting, the act of measuring and transferring small, precise volumes of liquid with a pipette. Pipetting is essential in molecular biology, chemistry, clinical diagnostics, and pharmacology. The pipetter’s duties include selecting the proper pipette and tip, setting the correct volume, pre-wetting the tip, aspirating and dispensing liquids with controlled speed, and performing serial dilutions or aliquoting as required. Pipetters must follow standardized protocols to ensure accuracy and reproducibility, and they are responsible for maintaining equipment and documenting measurements.

Common tools used by pipeters include micropipettes and adjustable-volume pipettes, with disposable plastic tips that prevent cross-contamination. Techniques emphasize consistent plunger travel, avoiding bubbles, and dispensing against the side of the vessel when appropriate. Temperature, liquid viscosity, and tip condition can affect results, so calibration and technique training are ongoing responsibilities.

Quality control involves regular calibration against reference standards, using gravimetric, colorimetric, or electronic verification methods, and keeping records for traceability. Safety considerations cover proper personal protective equipment, handling hazardous materials, and disposal of tips and liquids following institutional guidelines. In practice, pipetting accuracy depends on both the instrument and the operator’s adherence to established methods, making training and documentation central to the role of a pipetter.