Pipetting
Pipetting is the technique of measuring and transferring precise volumes of liquid using a pipette. Pipettes come in several types, including air-displacement micropipettes for small volumes (typically 1 μL to 1000 μL), positive-displacement pipettes for viscous or volatile liquids, and larger-volume devices such as serological or volumetric pipettes. Each pipette usually uses a disposable tip to prevent cross-contamination and to preserve accuracy.
Operation generally involves selecting a calibrated volume, attaching a disposable tip, pre-wetting the tip to improve
Calibration and quality control are essential to ensure reliable results. Regular calibration and verification of pipette
Pipetting is foundational in many laboratories, supporting applications in molecular biology, biochemistry, clinical testing, and pharmaceutical