KOHPräparation

KOHPräparation refers to a laboratory technique used to prepare samples for microscopy. It involves the use of potassium hydroxide (KOH), a strong base, to break down or soften certain biological tissues or structures. This process is particularly useful for making opaque or tough specimens more transparent, allowing for better visualization of internal details under a microscope.

The procedure typically involves immersing the sample in a solution of KOH, often at a specific concentration and temperature, for a defined period. The time and concentration of the KOH solution are adjusted based on the type of sample and the desired outcome. For instance, in mycology, KOHPräparation is commonly used to clear fungal elements, such as hyphae and spores, making them easier to identify and study. In some medical laboratories, it is employed to examine skin scrapings for the presence of fungi like *Candida* or dermatophytes.

The alkalinity of KOH is responsible for its action. It hydrolyzes the chitin and other complex carbohydrates found in cell walls of fungi and arthropods, as well as proteins in animal tissues. This breakdown process softens the material, making it pliable and more amenable to microscopic examination. After the KOH treatment, the sample is usually rinsed to remove excess KOH and then mounted on a microscope slide with a mounting medium, such as water or a staining solution, for observation. Care must be taken during the process, as KOH is corrosive and can cause skin and eye irritation.