Agrobacteriumbased
Agrobacterium-based transformation refers to techniques that use the bacterium Agrobacterium tumefaciens or related species to introduce foreign DNA into plant cells. The natural system centers on a Ti (tumor-inducing) plasmid that transfers a defined region called T-DNA into the plant genome, a process that causes crown gall disease. Researchers repurpose this mechanism to deliver genes of interest and produce stable, transgenic plants under appropriate biosafety controls.
Most widely used is the binary vector system, where a disarmed Ti plasmid supplies the virulence functions
Applications encompass a broad range of plant biotechnology goals, including crop traits such as pest resistance,
Advantages include relatively high transformation efficiency and a propensity for stable, single- or low-copy insertions with