34basepair

34basepair refers to a short double-stranded DNA fragment consisting of 34 base pairs. In practice it typically denotes a synthetic oligonucleotide duplex of this length, produced by chemical synthesis and used as a basic building block in molecular biology workflows. The term is length-based and applies to any sequence that yields a 34 bp duplex when paired with its complement.

Design considerations for a 34 base pair fragment include sequence uniqueness, stability, and specificity. Because 34 bp is relatively short, the sequence may occur in multiple places within a genome, so researchers often verify target uniqueness before use. Melting temperature, binding affinity, and potential secondary structures depend on the GC content and sequence context; moderate GC content (roughly 40–60%) and avoidance of long homopolymers or strong self-complementarity are commonly recommended. In applications such as primers or probes, a 34 bp length can offer higher specificity than shorter oligos but may require higher annealing temperatures and careful optimization of salts and additives.

Typical applications include use as oligonucleotide probes, barcode segments in sequencing libraries, or synthetic controls. They can serve as short primers or extension templates in various molecular assays, though longer primers are often preferred for standard PCR efficiency.

Synthesis and handling involve chemical synthesis of the oligonucleotides, followed by purification (for example, PAGE or HPLC) and storage under appropriate conditions, usually at low temperature. For double-stranded use, complementary strands are annealed under controlled cooling.

Limitations include limited sequence complexity and potential non-uniqueness in large genomes, underscoring the need for in silico validation before experimental deployment.