tiltseries
A tilt series is a sequence of two-dimensional images of a specimen acquired at progressively different tilt angles with respect to the imaging axis, typically collected in electron tomography using a transmission electron microscope. The series is used to reconstruct a three-dimensional volume of the sample, revealing its internal organization beyond what a single projection can show.
In practice, the specimen is tilted about a defined axis while images are recorded at discrete angles.
Alignment and reconstruction are core steps. Fiducial markers such as gold beads or feature-based tracking are
Variations include dose-symmetric tilt schemes that balance dose across tilts and continuous tilt acquisitions. Processing and
Applications of tilt-series tomography span structural biology, materials science, and related fields, enabling three-dimensional insight into