networkKnl1

networkKnl1 is a molecular interaction network centered on KNL1, a kinetochore scaffold protein essential for chromosome segregation. The network models how KNL1 coordinates microtubule attachment and spindle assembly checkpoint signaling through the KMN network and related regulators, integrating physical interactions and regulatory events during mitosis.

Core components include the KMN network (KNL1-Mis12-Ndc80), which forms the primary interface between kinetochores and microtubules. KNL1 contains MELT repeats that, when phosphorylated by Mps1, recruit Bub1-Bub3 to the kinetochore, initiating SAC signaling. The Mis12 and Ndc80 complexes contribute attachment stability and checkpoint amplification. Regulators such as Aurora B, PLK1, PP1, and SAC effectors (Mad1/Mad2, BubR1, Cdc20) are represented to reflect regulation and feedback.

Data sources and construction: networkKnl1 combines experimental interactions across species mapped to a common framework, emphasizing mitotic stage-specific contacts and phosphorylation events. It is intended as a resource for computational modeling and hypothesis generation rather than a complete catalog.

Applications: researchers use networkKnl1 to study kinetochore architecture, SAC activation, and error correction. It supports mitotic-timing simulations, exploration of drug targets for cancer, and cross-species comparisons.

Limitations: kinetochore signaling is dynamic and context-dependent, so the network remains a working model with incomplete data and species variation.