mNeonGreen
mNeonGreen is a monomeric green fluorescent protein used as a fluorescent reporter in live-cell imaging. It was engineered to be significantly brighter than enhanced GFP (EGFP) and to mature rapidly in mammalian cells. The protein is derived from a natural fluorescent protein found in reef coral species and has been optimized for high expression and stability across diverse organisms. As a monomer, it minimizes interference with the fused protein’s localization and function, and it exhibits strong photostability and a wide pH tolerance near physiological conditions.
Spectral properties and imaging uses: mNeonGreen has an excitation peak around 506 nm and an emission peak
Applications and availability: It is widely used in bacteria, yeast, plants, and mammalian cells, in fusion constructs