kryoEM
kryoEM, often written as cryo-EM, refers to cryogenic electron microscopy, a set of imaging methods used to determine the structures of biological macromolecules at near-native conditions. Specimens are rapidly frozen to form vitreous ice, preserving their structure without chemical fixation or staining. Using direct electron detectors and advanced software, kryoEM can reveal shapes and internal features at resolutions from a few tens of angstroms to sub-angstrom levels for suitable samples.
Two main modalities are single-particle cryo-EM (SPA) and cryo-electron tomography (cryo-ET). In SPA, purified macromolecules are
Sample preparation involves applying a small volume to a perforated grid, blotting to achieve a thin film,
Reconstruction converts many two-dimensional projections into three-dimensional maps, with resolution assessed by Fourier shell correlation. CryoEM