kollisionsinducerad

Kollisionsinducerad, also known as collision-induced dissociation (CID), is a technique used in mass spectrometry to fragment ions. This process is particularly useful in identifying the structure of molecules in complex mixtures. CID involves the collision of an ion with a neutral gas molecule, typically helium or nitrogen, in the gas phase. The collision transfers energy to the ion, causing it to break apart into smaller fragments. These fragments are then analyzed to provide information about the original molecule's structure.

The principle behind CID is based on the conservation of energy and momentum. When an ion collides with a neutral gas molecule, the kinetic energy of the ion is transferred to the molecule, leading to its fragmentation. The energy transferred can be controlled, allowing for the selective fragmentation of specific ions. This selectivity is crucial in mass spectrometry, where the goal is often to identify and quantify specific compounds in a mixture.

CID is commonly used in tandem mass spectrometry (MS/MS) experiments. In MS/MS, ions are first separated and identified by their mass-to-charge ratio (m/z) in the first mass spectrometer. These ions are then selected and fragmented using CID in the second mass spectrometer. The resulting fragments are then analyzed to provide detailed structural information about the original molecule.

The efficiency of CID depends on several factors, including the type of ion, the energy of the collision, and the nature of the neutral gas molecule. Higher collision energies generally result in more extensive fragmentation, but they can also lead to the loss of valuable structural information. Therefore, the energy of the collision is carefully controlled to balance the need for fragmentation with the need to preserve structural information.

In summary, kollisionsinducerad, or collision-induced dissociation, is a powerful technique in mass spectrometry that allows for the fragmentation of ions and the identification of molecular structures. Its principle is based on the transfer of energy from an ion to a neutral gas molecule, leading to the fragmentation of the ion. CID is widely used in tandem mass spectrometry experiments to provide detailed structural information about molecules in complex mixtures.