iDISCO
iDISCO stands for immunolabeling-enabled three-dimensional imaging of solvent-cleared organs. It is a tissue clearing technique that combines immunolabeling of intact biological specimens with solvent-based clearing to render tissues optically transparent and compatible with 3D fluorescence imaging. The method enables whole-organ labeling and imaging, allowing researchers to visualize cellular and molecular architecture within intact samples, such as the mouse brain, spinal cord, or other organs.
The iDISCO workflow typically begins with fixation of tissue, followed by permeabilization and immunostaining, using antibodies
A variant, iDISCO+, introduced to improve antibody penetration and labeling reliability in larger samples, often including
Limitations include potential tissue distortion from solvent exchange, loss or quenching of certain fluorophores, and the