furimazine
Furimazine is a synthetic luciferin substrate used by the enzyme NanoLuc luciferase to produce bioluminescence. It is a derivative of the luciferin class, designed to enable high brightness and robust assay performance. NanoLuc, a small luciferase originating from the deep-sea shrimp Oplophorus gracilirostris, catalyzes the oxidation of furimazine, emitting blue light with an emission peak near 460 nm. The reaction yields a very bright luminescent signal, supporting sensitive detection in a range of laboratory assays.
Chemically, furimazine is a stable, synthetic luciferin analog tailored to fit NanoLuc’s substrate pocket. It is
Availability and use: furimazine is marketed by Promega as part of the Nano-Glo luciferase substrate family,
Safety and handling: as with other chemical reagents used in bioluminescence assays, furimazine should be handled