UVspektroskopia

UV spektroskopia, also known as ultraviolet spectroscopy, is an analytical technique used to study the interaction between matter and ultraviolet (UV) radiation. This method involves measuring the absorption or emission of UV radiation by molecules, providing information about their electronic structure and behavior.

In UV spektroskopia, a sample is typically placed in a cell or solution and exposed to UV radiation from a lamp or other light source. The intensity of the radiation is then measured as a function of wavelength, often using a spectrophotometer or spectrometer. Analysis of the resulting spectrum reveals the presence of molecular species, their concentrations, and their interactions.

The technique is commonly used in organic chemistry to determine the structure and properties of compounds, particularly those with characteristic absorption bands in the UV region. In biochemistry, UV spektroskopia is applied to study the properties of biomolecules, such as nucleic acids and proteins.

There are several types of UV spektroskopia, including UV-visible spectroscopy, which covers both the UV and visible regions, and near-UV spectroscopy, which focuses on the region just beyond the visible spectrum. Other variations include fluorescence and phosphorescence spectroscopy, which detect the emission of radiation after excitation.

The technique has numerous applications in fields such as pharmaceuticals, where it is used to identify and monitor the purity of compounds, and environmental science, where it helps to detect pollutants and monitor water quality. Overall, UV spektroskopia has become a valuable tool in a wide range of scientific disciplines, enabling researchers to gather precise information about materials and systems at the molecular level.