TetRbased
TetRbased refers to regulatory systems that use the TetR family of transcriptional regulators to control gene expression in response to tetracycline-class inducers. These systems are widely used in synthetic biology and molecular biology to achieve inducible, reversible control of transcription across a range of organisms, including bacteria, yeast, and mammalian cells.
The core mechanism relies on a TetR repressor protein that binds to a specific operator sequence (tetO)
Common components of TetRbased systems include a TetR or rtTA/tTA repressor, tetO operator sites, and inducible
Applications span basic research, gene function studies, and therapeutic contexts, where precise, tunable control of expression