SpleII

SpleII is a restriction enzyme, a type of endonuclease that recognizes and cuts specific DNA sequences. It is derived from the bacterium Sphingomonas paucimobilis, which is a Gram-negative bacterium commonly found in soil. SpleII is part of the Type IIS restriction enzyme family, which includes enzymes that recognize palindromic sequences of DNA. The recognition site for SpleII is a 12-base pair palindrome, with the sequence 5'-CCCGGACCGGG-3'. This enzyme is often used in molecular biology and biotechnology for various applications, including DNA cloning, gene editing, and the construction of recombinant DNA molecules. SpleII is notable for its high specificity and efficiency in cutting DNA at the recognition site, making it a valuable tool in genetic research and engineering. The enzyme is typically isolated from the bacterium and purified for use in laboratory settings.