SECSAXS

SECSAXS, or Small-Angle X-ray Scattering with Size-Exclusion Chromatography, is a powerful analytical technique used primarily in the field of structural biology and biochemistry. This method combines the capabilities of size-exclusion chromatography (SEC) with small-angle X-ray scattering (SAXS) to provide detailed information about the shape, size, and structural properties of macromolecules in solution.

Size-exclusion chromatography is a type of liquid chromatography that separates molecules based on their size. It is particularly useful for purifying and characterizing proteins, nucleic acids, and other biomolecules. Small-angle X-ray scattering, on the other hand, involves the scattering of X-rays by a sample to produce a pattern that can be analyzed to determine the sample's structural features at the nanometer scale.

In SECSAXS, the sample is first separated by size-exclusion chromatography, and the eluted fractions are then subjected to small-angle X-ray scattering. This combination allows for the study of individual components within a complex mixture, providing high-resolution structural information without the need for crystallization or other preparative steps. The technique is non-destructive and can be applied to a wide range of biological samples, including proteins, nucleic acids, and their complexes.

SECSAXS is particularly valuable for studying the dynamics of biomolecular interactions, conformational changes, and the overall architecture of macromolecular assemblies. It is widely used in research settings to gain insights into the structural biology of various biological systems, contributing to our understanding of molecular mechanisms and functions. The integration of SEC with SAXS enhances the resolution and accuracy of structural data, making SECSAXS a versatile and informative tool in modern biochemistry and structural biology.