OPLpath2

OPLpath2 is an open-source software tool designed for the analysis of optical projection tomography (OPT) data. Developed as a successor to OPLpath, it is primarily used in biological and medical imaging research to reconstruct three-dimensional images from optical projection data. The software is particularly well-suited for small animal imaging, such as whole-body scans of rodents, where it helps visualize internal structures with high resolution.

OPLpath2 operates by processing raw projection images acquired through OPT, which involves capturing light transmitted through a specimen at various angles. The software employs advanced algorithms to reconstruct these projections into detailed volumetric images, allowing researchers to study tissue morphology, vascular networks, and other biological features. It supports various imaging modalities, including fluorescence and brightfield microscopy, making it versatile for different experimental setups.

The tool is written in MATLAB, a widely used programming language in scientific computing, and is distributed under an open-source license, enabling customization and collaboration among researchers. OPLpath2 includes features for image preprocessing, reconstruction, and visualization, with options for adjusting parameters to optimize results based on specific experimental needs. It also integrates with other imaging software, facilitating seamless workflows in research laboratories.

OPLpath2 has been utilized in studies involving cancer research, developmental biology, and neuroimaging, among others. Its ability to provide high-resolution, three-dimensional reconstructions makes it a valuable resource for advancing our understanding of biological systems. The software is continuously updated to incorporate new algorithms and improvements, ensuring its relevance in evolving imaging technologies.