MINFLUX

MINFLUX is a cutting-edge fluorescence microscopy technique developed to achieve nanometer-scale localization of fluorescent molecules with minimal photon expenditure. The method was introduced to overcome limitations of conventional super-resolution techniques such as STORM and PALM by combining the principles of minimal photon use with precise spatial localization.

The core principle of MINFLUX involves the use of a targeted laser beam to excite fluorescent molecules while simultaneously applying a spatially structured light field, typically a doughnut-shaped beam with a central zero-intensity point. By carefully positioning this zero-intensity point and measuring the fluorescence signal, researchers can determine the molecule's position with exceptional precision. This approach allows for high localization accuracy with significantly fewer emitted photons, reducing photobleaching and enabling longer imaging times.

MINFLUX has demonstrated the ability to localize molecules with nanometer precision in both two-dimensional and three-dimensional space. It is especially beneficial for studying live-cell dynamics and biological processes requiring high spatial and temporal resolution. The technique's efficiency and precision have made it a valuable tool in molecular and cellular biology, advancing understanding at the single-molecule level.

Developed by researchers including Stefan W. Hell, who pioneered super-resolution fluorescence microscopy, MINFLUX represents a significant advancement in the field. Its continuous development aims to extend capabilities such as increased imaging speed, higher spatial resolution, and adaptation for broader biological applications. As of now, MINFLUX remains an active area of research with ongoing improvements to optimize its performance and accessibility.