LSALSI

LSALSI, or Least Squares Adaptive Light Source Imaging, is a technique used in microscopy to improve the resolution and contrast of images. It is particularly useful in fluorescence microscopy, where the signal from the sample is often weak and noisy. LSALSI works by adaptively adjusting the illumination pattern of the microscope to optimize the signal-to-noise ratio of the image. This is achieved by iteratively solving a least squares problem, which minimizes the difference between the measured and expected signals. The technique can be applied to both widefield and confocal microscopes, and has been shown to improve the resolution of images by up to a factor of two. LSALSI is a powerful tool for improving the quality of microscopy images, and has been used in a variety of applications, including live-cell imaging and super-resolution microscopy. However, it requires specialized hardware and software, and may not be suitable for all microscopy applications.