Immunoblottings
Immunoblottings, commonly referred to as Western blotting, are a set of methods used to detect specific proteins in a complex mixture after separation by gel electrophoresis and transfer to a membrane.
In the standard approach, proteins are denatured and separated by size on an SDS-PAGE gel, then transferred
The position on the membrane reflects molecular weight, and signal intensity provides an estimate of relative
Applications include verification of protein expression, analysis of post-translational modifications, and detection of specific antigens in
Limitations include dependence on antibody specificity and potential non-specific bands; the technique is semi-quantitative and sensitive
The method emerged in the late 1970s and early 1980s, with foundational work by Towbin and colleagues;