FörsterResonanzenergietransfer
Förster Resonance Energy Transfer, often abbreviated as FRET, is a non-radiative energy transfer mechanism between two light-sensitive molecules called chromophores. It occurs when a donor fluorophore in its excited electronic state transfers energy to an acceptor chromophore through long-range dipole-dipole interactions. This transfer is highly dependent on the distance between the donor and acceptor, typically occurring over distances of 1 to 10 nanometers. The efficiency of FRET decreases with the sixth power of the distance, making it a sensitive probe for molecular proximity.
The process requires that the emission spectrum of the donor overlaps significantly with the absorption spectrum
FRET finds widespread applications in various scientific fields, particularly in molecular biology and biophysics. It is