DpnII
DpnII is a type II restriction endonuclease enzyme that is commonly used in molecular biology for DNA cleavage and cloning. It is derived from the bacterium Deinococcus radiodurans and is part of the Dpn family of restriction enzymes. DpnII recognizes a specific DNA sequence, 5'-GATC-3', and cleaves the DNA at this site, creating blunt ends. This enzyme is particularly useful in cloning applications because it does not cut methylated DNA, allowing for the insertion of foreign DNA into a methylated vector without self-cleavage.
DpnII is typically used in conjunction with other restriction enzymes to create unique restriction sites for
In addition to its use in cloning, DpnII has been studied for its potential applications in biotechnology