DNAaduktitesta

DNAaduktitesta, also known as the Comet assay, is a widely used technique in molecular biology and toxicology to assess DNA damage and repair. It is based on the principle that damaged DNA migrates more slowly through an electrophoretic field than undamaged DNA. The assay involves suspending cells or isolated nuclei in a low-melting-point agarose gel, which is then lysed to release the DNA. The DNA is then subjected to electrophoresis, and the resulting DNA fragments are visualized under a fluorescence microscope. The extent of DNA damage is quantified by measuring the tail length or percentage of DNA in the tail, which represents the damaged DNA. The Comet assay is highly sensitive and can detect a wide range of DNA lesions, making it a valuable tool for studying the effects of environmental toxins, radiation, and other DNA-damaging agents. It is also used in clinical settings to monitor DNA damage in patients with genetic disorders or cancer. The Comet assay is relatively simple, inexpensive, and can be performed on a wide range of cell types, making it a popular choice for DNA damage research.