DNAFiberAnalysen
DNAFiberAnalysen, or DNA fiber analysis, refers to microscopy-based methods that study individual DNA molecules stretched into elongated fibers on a solid substrate. By spreading single DNA molecules into linear fibers and labeling them with fluorescent probes, researchers visualize features along the molecule with high physical resolution. The main approaches include molecular combing and related fiber-stretching techniques, often combined with fluorescent in situ hybridization (FISH) or sequential nucleotide labeling.
In replication assays, cells are labeled sequentially with nucleotide analogs such as IdU and CldU. After spreading
Applications include studying replication dynamics under normal and stressed conditions, DNA repair pathways, and structural variation,
Limitations include low throughput, labor-intensive sample preparation, and potential artifacts from stretching and drying. Resolution typically
History and development: DNA fiber analysis emerged in the late 1990s and early 2000s to study replication