DH10B

DH10B is a commonly used laboratory strain of Escherichia coli designed for molecular cloning and plasmid propagation. It is widely adopted in academic and industrial settings for its high transformation efficiency and reliable plasmid maintenance, making it a standard choice for cloning workflows, library construction, and routine recombinant DNA work.

The strain is characterized by genetic modifications intended to enhance cloning performance. Features typically include mutations that reduce unwanted recombination and improve plasmid DNA quality, contributing to stable propagation of recombinant constructs and larger inserts. DH10B is commonly supplied by multiple vendors and serves as a versatile host for plasmid amplification, verification, and downstream applications such as sequencing or expression screening.

Applications of DH10B span general cloning, preparation of size-selected plasmid libraries, and other tasks that require efficient uptake of DNA and stable maintenance of plasmids in E. coli. It is frequently used in conjunction with standard molecular biology techniques, including transformation, plating, and plasmid isolation.

Culturing DH10B follows routine bacteriology practices. The strain is typically grown on media appropriate for Escherichia coli at standard incubation temperatures, using conventional antibiotic selection when plasmids or markers are present. As with other cloning strains, safety and handling conform to standard BSL-1 laboratory guidelines.

See also: DH5α, TOP10, cloning strains of Escherichia coli, plasmid propagation.