Caco2solumallit
Caco-2 solumallit, or Caco-2 cell models, are in vitro models of the human intestinal epithelium derived from a colorectal adenocarcinoma cell line. When cultured on permeable inserts, Caco-2 cells differentiate into a polarized monolayer with tight junctions, microvilli and brush-border enzymes, closely resembling enterocytes of the small intestine. They are widely used to study oral drug absorption, intestinal permeability and transporter activity.
Typical setup involves growing cells on Transwell inserts, creating distinct apical and basolateral compartments. Barrier integrity
Applications and enhancements: Caco-2 models are used to screen drug permeability, predict oral bioavailability and support
Limitations: They do not fully replicate the complexity of the in vivo intestine, lacking microbiota and dynamic