qPCRi
qPCRi, also known as quantitative PCR inhibition, refers to a phenomenon where the efficiency of quantitative polymerase chain reaction (qPCR) is reduced or completely prevented. This inhibition can lead to inaccurate quantification of nucleic acid targets, impacting experimental results. Several factors can cause qPCR inhibition. These include the presence of inhibitors carried over from the sample preparation process, such as polysaccharides, humic acids, or certain salts found in environmental or plant samples. Inhibitors can also be introduced through reagents or consumables used in the experiment.
The mechanism of inhibition often involves interference with the enzymatic activity of the DNA polymerase. Inhibitors
Detecting and mitigating qPCR inhibition is crucial for reliable results. Common strategies include optimizing sample purification