pMB1ColE1derived

pMB1ColE1derived refers to a family of plasmids whose replication origin is derived from the pMB1-ColE1 replicon. These plasmids are commonly used in Escherichia coli for high-copy cloning and expression applications. The pMB1 component is a derivative that enhances copy number relative to the parental ColE1 origin, resulting in elevated plasmid yields in many laboratory strains.

Replication and copy number are governed by ColE1-type control mechanisms, with the pMB1-derived elements contributing to a higher-copy phenotype. In practice, pMB1ColE1-derived plasmids are considered high-copy plasmids, and their exact copy number can vary with host strain, growth conditions, and metabolic burden.

Genetic features typically include a selectable marker, such as an antibiotic resistance gene, and a multiple cloning site for easy insertion of DNA fragments. These plasmids are widely employed for cloning, high-level protein expression, and the construction of DNA libraries, taking advantage of their rapid propagation and increased yield of recombinant material. Their modular design often allows swapping of resistance markers and inserts to fit specific experimental needs.

Compatibility and safety considerations center on the replication origin, which influences plasmid compatibility with other vectors in a single host. pMB1-ColE1-derived plasmids may be incompatible with other ColE1-based plasmids or closely related origins, so planning plasmid combinations is important. As with all genetic tools, these plasmids should be used in accordance with relevant biosafety and institutional guidelines.

See also: ColE1 origin, pMB1 origin, pUC plasmids, high-copy plasmids.