jRGECO

jRGECO is a genetically encoded fluorescent protein that emits red fluorescence. It is a derivative of the coral fluorescent protein RGECO, which was originally engineered for improved phot performance and pH insensitivity. jRGECO, developed by Dr. Loren Looger and colleagues at Janelia Research Campus, offers distinct spectral properties and enhanced brightness compared to its predecessors.

The excitation and emission peaks of jRGECO are approximately 550 nm and 580 nm, respectively, placing its fluorescence in the orange-red region of the spectrum. This spectral profile makes it suitable for various biological imaging applications, particularly when combined with other fluorescent proteins that emit at different wavelengths. jRGECO is often used for live-cell imaging, allowing researchers to visualize cellular structures and processes in real-time. Its relatively fast maturation and good photostability contribute to its utility in dynamic imaging experiments.

Researchers utilize jRGECO for a range of studies, including tracking protein localization, monitoring gene expression, and analyzing cellular signaling pathways. Its development has expanded the toolkit available to scientists for multicolor imaging and fluorescence microscopy, enabling more complex and detailed investigations into biological systems. Like other genetically encoded fluorescent proteins, jRGECO is expressed in cells by introducing the gene encoding the protein through genetic manipulation techniques.