fluoresenssikontaktia

Fluoresenssikontaktia, often translated as fluorescence immunoassay or fluorescent immunoassay, is a laboratory technique used to detect and quantify specific substances, typically antigens or antibodies, in a sample. It relies on the principle of fluorescence, where a substance emits light of a specific color when exposed to ultraviolet or visible light.

In a typical fluoresenssikontaktia assay, a specific antibody or antigen is labeled with a fluorescent molecule called a fluorophore. This labeled molecule is then allowed to interact with the sample. If the target substance is present in the sample, it will bind to the labeled molecule, forming an antigen-antibody complex.

After the binding reaction, the sample is washed to remove any unbound labeled molecules. The presence and amount of the bound fluorescently labeled molecules are then measured using a fluorometer or a specialized fluorescence reader. The intensity of the emitted fluorescence is directly proportional to the concentration of the target substance in the original sample.

Fluoresenssikontaktia is a highly sensitive and specific method, making it valuable in various diagnostic and research applications. It is widely used in clinical diagnostics for detecting infectious diseases, hormones, drugs, and tumor markers. In research, it is employed for protein analysis, cell imaging, and gene expression studies. The technique's versatility allows for adaptation to different formats, including qualitative detection and quantitative measurements.