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apochromaten

An apochromat (plural apochromats) is a high-performance microscope objective designed to correct chromatic and spherical aberrations across a broad portion of the visible spectrum. Compared with standard achromats, apochromats minimize lateral and axial chromatic error for multiple wavelengths, resulting in higher sharpness, improved color fidelity, and closer to diffraction-limited performance.

The correction of three or more wavelengths is a defining feature. Apochromats typically use multiple optical

Applications include fluorescence and multicolor imaging, where accurate alignment of different fluorophores is crucial, as well

Limitations include higher cost, greater physical size and weight, and increased sensitivity to misalignment or refractive-index

elements
made
from
low-dispersion
or
special
glasses,
and
may
include
fluorite
(CaF2)
or
other
high-quality
materials
to
reduce
dispersion.
Many
apochromats
are
also
“plan”
objectives,
meaning
they
are
corrected
for
flat
field
curvature
so
image
planes
remain
sharp
across
the
field
of
view.
They
are
commonly
offered
in
oil-immersion
variants
with
high
numerical
apertures
(often
around
1.0
to
1.4)
and
are
designed
to
be
used
with
correctly
matched
cover
slips
and
immersion
media.
as
quantitative
imaging,
confocal
microscopy,
and
some
super-resolution
setups.
The
superior
correction
properties
make
apochromats
particularly
valuable
in
experiments
requiring
faithful
color
rendering
and
precise
measurement
of
axial
and
lateral
distances.
mismatches
in
samples.
Proper
matching
of
immersion
medium,
cover-slip
thickness,
and
mounting
media
is
essential
to
realize
the
performance
advantages
of
apochromats.